Oligonucleotides
are synthesized on the Applied Biosystems 394 DNA/RNA Synthesizer. Oligonucleotides
sequences can be produced up to 150 nucleotides in length at various
synthesis scales which produce up to milligram quantities of high-quality
Oligonucleotides for use as primers, probes or other applications. The
synthesized oligonucleotides can be purified by Oligonucleotides Purification
Cartridge(OPC). Specialized nucleotides can be added to the sequences
as well as labeling of the oligos with fluorescent or other derivatives.
The NJMS-MRF synthesizes oligonucleotides at the following scales: 200
nmole, 1 µmole. Our delivery time is 1 to 2 working days for the
200-nmole scale for the synthesis of up to 50 bases. Long oligos (up
to 150 mers) and a wide variety of modified oligos and doped oligos
are available.
Our Guarantee:
We guarantee the
quality of our oligos. Our policy: If there is a problem with an oligo
we synthesize, we will resynthesize the oligo at no charge.
Oligonucleotide
properties calculator
Oligonucleotide
Properties Calculator
Fluorescence
Properties of Available Dyes
| |
Absorbance
Maximum |
Emission
Maximum |
Quencher |
Cost
per Lable* |
| Fluorescein
(6-FAM) |
494nm |
525nm |
BHQ-1 |
$25 |
| TET |
521nm |
536nm |
BHQ-1 |
$75 |
| HEX |
535nm |
556nm |
BHQ-1 |
$75 |
| TAMRA |
565nm |
580nm |
BHQ-2 |
$110 |
| CY3 |
552nm |
570nm |
BHQ-2 |
$75 |
| CY3.5 |
588nm |
604nm |
BHQ-2 |
$110 |
| CY5 |
643nm |
667nm |
BHQ-3 |
$75 |
| CY5.5 |
683nm |
707nm |
BHQ-3 |
$110 |
| CAL
Fluor Gold 540 |
522nm |
544nm |
BHQ-1 |
$75 |
| CAL
Fluor Orange 560 |
538nm |
559nm |
BHQ-2 |
$75 |
| Quasar
570 |
548nm |
566nm |
BHQ-2 |
$60 |
| CAL
Fluor Red 590 |
569nm |
591nm |
BHQ-2 |
$80 |
| CAL
Fluor Red 610 |
590nm |
610nm |
BHQ-2 |
$75 |
| CAL
Fluor Red 635 |
618nm |
637nm |
BHQ-2 |
$80 |
| Quasar
670 |
647nm |
667nm |
BHQ-3 |
$60 |
* Probes are synthesized at the 200 nm scale at 0.65
per base, $20 for the BHQ and $25-$110 for the label.
